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The range of detection for validation samples was ND to 115 pg ml−1; 24% were detected with a mean result of 61 pg ml−1.
The range of detection for validation samples was 6635 13 553 pg ml−1 with a mean result of 9577 pg ml−1.
In the microarray datasets used for validation, samples with RIC8A signal intensity < 650 were considered as low expressed and RIC8A signal intensity > 950 as highly expressed.
In this analysis, the difference in TTRs between the four clusters was still significant (P-value 0.045) with the median TTR of 512 days for validation samples assigned to cluster 1 but not reached for the remaining clusters (Fig. 5a).
Levels of non-GM contamination were 0.1, 0.5, 1.0, 2.0, 5.0 and 10.0% (weight/weight) for standard samples and 1.0, 2.0, 4.0 and 8% (weight/weight) for validation samples.
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The strict application of the colourant assignment criterium results on 100% of correct classifications for all validation samples.
Validation samples For the validation samples, fresh WB was collected in BD Vacutainer® fluoride/oxalate tubes from two healthy donors and aliquots were stored at −20 °C.
Furthermore, we conducted Sanger sequencing on the coding regions of several genes for the validation samples.
The mean intra-assay imprecision (%CV) for these validation samples ranged from 1 to 18% (Additional file 1, Table S6).
The gEBV accuracy generally increased with increasing heritability of the trait, corresponding to an increase in the mean pEBV accuracy for the validation samples (Table 3).
For the validation samples, miRNAs were extracted from 200 μl serum spiked with 25 fmol synthetic cel-miR-39 and eluted in 100 μl RNase-free water.
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