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We ruled out the possibility of STZ-induced toxicity by administering insulin for validation experiment (as depicted in Figure 1) following STZ-treatment and observed recovery in axonal transport rates.
To avoid false-positive finding due to mass significance, 10 more gels using IPG 3 10NL and prepared from a separate experiment involving five tumour-bearing mice and five free-fed control mice (n=5 per group) were examined (one gel per animal) and used for validation (experiment 2; Figure 2; Supplementary Figure 4).
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Validity of the models have been checked through experiments and the overall error percentages of surface roughness (Ra) and micro-turning depth deviation for validation experiments are 2.83 and 2.96%, respectively.
We selected three genes from the trans8_eQTL signature for validation experiments in knockout mice.
In total 34 CSF samples were used for mass spectrometry analysis, while twenty of these samples and twenty additional samples were used for validation experiments.
For validation experiments we selected UniGene clusters composed of three or more GenBank sequences (Tables S2 and S4), excluding Hs.551051 and Hs.617329 that required further analysis to clarify whether they represent a single gene.
These estimates of hypothetical changes of gene expression were used solely for purpose of prioritizing predicted targets and narrowing down list of predictions in order to select those that are most likely to be affected in experimental groups of animals and would be more reliable candidates for validation experiments.
Also it is the 1000GP standard for validation experiments.
bSample used for validation experiments, but not microarray analysis.
An additional 9 AML and 10 PBMC samples were used for validation experiments.
Moreover, the prediction profile likelihood concept is extended to obtain confidence intervals for validation experiments.
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