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The independent VAS variables were selected for two subsequent steps in the regression analysis.
These single-conformer models and associated electron density maps were used as input for two subsequent steps.
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The procedure consists of two subsequent steps.
The treatment consisted of two subsequent steps: the first one using H2SO4 1% (v/v), to remove hemicellulose mainly, and the second one with NaOH for delignification.
Following the focusing step, the proteins on the strip were reduced and alkylated with gentle shaking in two subsequent steps for 30 min each in 10 ml equilibration buffer (6 M Urea, 75 mM Tris HCl pH 8.8, 29.3% glycerol, 2% SDS, 0.002% bromophenol blue) containing 100 mg DTT and 250 mg iodoacetamide in the second step.
To select for statistical significance of the signal ratios, two subsequent steps were applied.
The presented time-in-transformations-approach consists of three subsequent steps.
The study has been carried out using two subsequent steps.
The construction of the library was done in two subsequent steps.
In this paper, development and simulation of the model comprises two subsequent steps.
Statistical analyses were carried out in two subsequent steps following a logistic regression approach.
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