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Here we report that cardiac dysfunction induced by high-fat-diet (HFD) persists for two subsequent generations in Drosophila and is associated with reduced expression of two key metabolic regulators, adipose triglyceride lipase (ATGL/bmm) and transcriptional cofactor PGC-1.
We then reared individuals under isolated or crowded conditions for two subsequent generations.
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These lines were continued through single seed descent for six subsequent generations, resulting in approximately 500 RILs (after line loss) in the F8 generation, used in the work described herein.
Phenotypes were scored for both the injected worms and two subsequent generations on a daily basis.
Validation data included phenotyped and genotyped birds from five subsequent generations (on average 306 birds/generation).
The researchers analyzed RNA from recently activated T cells and two subsequent generations.
We first tested two subsequent generations of students on their second year and then we tested one generation after one year and another after two years.
The pedigree line underwent two subsequent generations of conventional selection, whereas the genomic line underwent four generations of GS.
Linkage disequilibrium was analyzed using the SNP data for chromosomes 1 and 4 on roosters from the two lines from two consecutive years (2003 and 2004), reflecting two subsequent generations.
All animals retained widespread homogenous fluorescence for at least five subsequent generations.
The fungicide vinclozolin induces fertility problems and abnormalities in rats for at least four subsequent generations.
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