Sentence examples for for two hr from inspiring English sources

Plates were rewashed and reacted for two hr with 100 µL of 1 µg/mL biotinylated detection anti-PA antibody (8240, Abcam, Cambridge, MA).

Rat BMDM were seeded in 96 well plates, allowed to adhere, and infected with subsp. novicida, LVS, subsp. holarctica and subsp. tularensis for two hr, as above.

Cultures were fixed in 4% paraformaldehyde in PBS and then incubated with the primary antibodies in blocking buffer (PBS containing 5% goat serum, 2% BSA, and 0.2% Triton X-100) at room temperature for two hr.

Rat BMDM were seeded into 96 well plates (2×105 cells/well), allowed to adhere, and then infected (10 and 100 MOI) with subsp. novicida, LVS, subsp. holarctica and subsp. tularensis for two hr to allow phagocytosis of bacteria.

To compare gross phagocytic ability, macrophages were seeded in 6 well tissue culture plates (1×106 cells/well) and incubated for two hr with fluorescently labeled microbeads at a concentration of either 10 or 100 beads per cell.

Cells were allowed to adhere and spread for two hr.

The tubes were left at −20°C for two hrs and then centrifuged at 8500 rpm at 4°C.

The flasks were incubated for two hrs at 25°C with shaking at 200 RPM, then at 42°C for 8 hrs, and held at 4°C without shaking until the following morning.

Gels were gently agitated for 30 min at room temperature in a filtered staining solution (20% methanol; 2% glycerol; 0.05% Toulidine Blue), then destained for two hrs with several changes of the same solution without dye.

For immunoblotting analysis with IIH6C4, MANDAG2-7D11, anti-c-Myc-Myc antibodies, PVDF membranes were blocked with 3% BSA in TBST (50 mM Tris, pH 7.4, 150 mM NaCl, 0.05% Tween-20) for 30 min and incubated with primary antibodies in TBST and 3% BSA for two hrs.

To further rule out the possibility that the protective effect of rapa on cell death is linked to its G1 function, cdc13-1 cells were pre-incubated at the non-permissive temperature (37°C) for two hrs to allow the majority of cells (greater than 95%) to exit G1 and accumulate at the G2/M phase (Fig. 2A).