Sentence examples for for two hours subsequently from inspiring English sources

Antonaglia and colleagues [ 58] ventilated a series of patients with severe exacerbation of COPD using PSV delivered by face mask for two hours; subsequently, only those in whom gas exchange improved were randomized to helmet or face mask.

On day 1 post isolation, monocytes were pre-incubated with Vpx-supplemented or non-supplemented PBj VLPs at an MOIeq of 1 for two hours, and subsequently infected with the CCR5-tropic HIV-1 virus strain SF162 at an MOI of 0.5.

Monocytes were pre-incubated at day 1 post isolation with PBj-derived VLPs either containing or lacking Vpx at an MOIeq of 1 for two hours and subsequently infected with the CCR5-tropic HIV-1 virus strain SF162 at an low MOI of 0.5.

After drug treatment, cells were incubated with 5 mg/ml MTT for two hours, and subsequently solubilized in DMSO.

MPO was also reacted with increasing hydrogen peroxide concentrations for two hours and subsequently resolved by SDS PAGE under non-reducing condition (Fig. 4D upper panel).

The gel was incubated in the presence of 2.5% Triton X-100 at room temperature for two hours and subsequently at 37°C over might in 10 mM CaCl2, 0.15 M NaCl, and 50 mM Tris (pH 7.5).

Protein A-coated 5.5 µm beads (Bangs Laboratories, Inc., Fisher, IN) were incubated with hE-Fc or anti-HLA antibodies in binding buffer (25 mM Boric acid, 150 mM NaCl, 1 mM CaCl2, 0.5 mM MgCl2, and 0.001% Tween 20, pH 8.0) for two hours at 4°C, and subsequently blocked with human Fc fragment in 1% BSA in PBS++ (PBS supplemented with 1 mM CaCl2, 0.5 mM MgCl2) for 1 hr at 4°C.

After four weeks of diet intervention, mice were fasted for five hours and subsequently anaesthetized with a mixture of isoflurane (1.5%), nitrous oxide (70%), and oxygen (30%).

Therefore, Vector, VDRff and VDRFF cells were treated with 1,25D3 for twenty four hours and subsequently subjected to co-immunoprecipitation using a rabbit polyclonal antibody against RXRα.

Twenty four hours post-transfection, cells were incubated in the presence or absence of 100 nM 1,25D3 for twenty four hours and subsequently assessed for the firefly and Renilla luciferase activities using the Dual- Luciferase Reproter Assay Kit (Promega, Madison, WI).

Thus, in order to examine the effects of persistent hyperdopaminergia on baseline HTOs, DAT-KO and WT mice were habituated to the recording chamber for eight hours and subsequently subjected to two-hour continuous electrophysiological and behavioral recordings.