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LigB9-11 alshowedwed high-affinity binding to collagen type III with an estimated Kd of 30 nM (range 10 nM for two determinations) (Figure 2B).
Average values were calculated for two determinations made on a single aliquot of serum from each animal [ 8].
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was 5.53% for eight determinations.
The precision for six determinations (n = 6) of 0.02 and 0.20 mg L−1 Hg II) was 4.1 and 3.5% (relative standard deviation), respectively.
Limits of detection were found to be 0.25 and 0.57 μmol L−1 for UA and AC, respectively, and the precision assessed as relative standard deviation (RSD) for ten determinations was found to be 3.20% and 1.34% for respective concentrations of 10.0 and 72.5 μmol L−1 for UA and 4.3% and 1.6% for AC.
a Mean and RSD% for five determinations b Theoretical values of F and t for p = 0.05 and n = 5 are 6.39 and 2.31, respectively.
The data include method accuracy and precision represented by the relative error (RE) and RSD, respectively, for six determinations at each concentration level.
Table 2 Determination of FA in commercial formulation by the proposed and official methods Concentration (20 ng/mL) Recovery % a ( ± RSD) Proposed method Official method Tablets 99.31 ± 0.30 99.56 ± 0.23 t- 0.29 (2.30) b F- 1.74 (6.38) b Recoveryc (%, ± RSD) 99.24 ± 0.34 a Mean Recovery % and RSD for five determinations.
This was also confirmed in the present study, in which a recovery value (expressed as the mean percentage recovery ± SEM for three determinations) of 90.5 ± 3.8 was observed with the above acetaldehyde concentration.
A combined nitric oxide formation (determined by binding to myoglobin) and NADPH molecules consumption assay [ 24] showed partial decoupling of the enzyme preparations, with 5.1±1.6 NADPH molecules consumed in the formation of 1 nitric oxide molecule by Physarum NOS A and 5.9±1.5 NADPH molecules consumed per nitric oxide molecule in Physarum NOS B (mean±S.D. for three determinations).
Blood samples for methadone determination were obtained once the patient was enrolled for 4 months at the MMT and dose was stabilized (according to inclusion criteria) in the MMT for 2 months.
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