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Hippocampal ACh levels were elevated while the rats were confined to the shock-probe chamber and remained elevated for two additional sample periods after the rats were returned to the microdialysis bowl (Figure 1a).
Prior to qRT-PCR, 80 ng of total RNA was reverse transcribed from each biological replicate used for the microarray study along with two additional samples (total n = 6 per experimental condition) using the QuantiScript Reverse Transcriptase kit (Qiagen, Valencia, CA) according to manufacturer instructions.
Seventy-one samples were sequenced for MHC class I and 59 of 71 samples used in the MHC analysis were also sequenced for ND2 along with five additional samples.
We administered this provisional survey to three additional samples of managers.
The process was repeated for two additional operators; two samples were centrifuged in the ZIPocrit and five samples were centrifuged in the hand-powered centrifuge for each of the two additional operators.
Gene expression assays were performed using the same sample set for technical validation and five additional samples (from five different individuals) for biological validation.
For this reason we included in the experimental setup three additional samples for analysis during the same time period.
In total 34 CSF samples were used for mass spectrometry analysis, while twenty of these samples and twenty additional samples were used for validation experiments.
In addition, fifty samples from a control group and twenty-five additional samples from dental nurses were taken for comparison.
In the male groups, three additional samples were added for controls and PD, respectively.
Three additional samples (Bat/KE5096/07, Bat/KE5105/07, and Bat/K5175/07) were positive for rotavirus.
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CEO of Professional Science Editing for Scientists @ prosciediting.com