Sentence examples for for two additional hours from inspiring English sources

The obtained suspension was magnetically stirred for two additional hours at 80 °C.

The culture was diluted 1 10 in fresh 3.7% BHI and incubated for two additional hours to reach log-phase growth.

50 µl of Dynabeads® M-270 Streptavidin (Dynal Biotech, Invitrogen) pre-washed twice and pre-equilibrated for 2 h at 4°C with blocking buffer (base buffer with 100 mM NaCl, 100 units/ml RNasin, 0.2 mg/ml tRNA, 0.1 mg/ml glycogen, 10 mM BSA and 2 mM DTT) was added to the binding mixture and incubated for two additional hours.

For cytotoxicity assays, C-1330 concentranging ranging from 10 nM to 100  μM were added for two additional hours.

Time-dependent β-galactosidase activities in the three strains are shown in Figure 3. Activities were barely detectable at time zero but increased after the first hour and for two additional hours before reaching a plateau.

(B ) Cells were incubated at 16°C for 2 hr in the presence of the disaggregating drug, then the temperature was shifted to 20°C for two additional hours in the absence of the drug.

The temperature was reduced to 16°C for 1 h at which time recombinant SpGH38 was induced by the addition of 0.2 mM isopropyl 1-β-D-thiogalactopyranoside and incubated for sixteen additional hours.

"As a courtesy for the inconvenience, please accept a voucher for three additional hours of your life, redeemable upon your death".

As part of the agency's contract with its operators, if a driver works on his or her birthday, he or she is automatically credited for eight additional hours.

Expression of target proteins was induced by nisin (see Results part for dilution used; for every round of nisin production, systematic tests were performed to determine optimal dilutions required for production of target proteins) and cultures were incubated for four additional hours.

Cells were grown at 37°C until OD600 nm 0.5 then induced with 0.5 mM IPTG for five additional hours.