Sentence examples for for total expression from inspiring English sources

Receptor cell surface expression and total receptor expression was determined by flow cytometry using a fluorescence signal detected from cells pre-incubated with the fluorescent probe in the absence (For surface expression % and surface density values) or presence of a mild detergent (For total expression % and total density values), respectively.

The two sample sets gave similar expression profiles in response to SSR, thus validating the data (Fig. 4C shows mean values for total expression data for the two sample sets).

The order of C-terminal tags to promote soluble expression was similar for total expression: MBP-H10 > GST-H10 > V5-H6 > Dhfr-H10 ~ GFP-H10 ~ Trx-H10 > H10 ~ DHFR-H10.

In support of the ASE data, the regression slopes for total expression (a coarser metric than ASE) differ between heterozygotes and homozygotes for both genes, significantly so for Hbb-b2 (p=0.04).

Where alternatively expressed isoforms were present in the region of interest, inventoried Taqman Assays were purchased (Life Technologies, Foster City, USA), information for which is shown in Additional file 2. Assays for total expression of all wild-type transcripts as well as the novel transcripts were also purchased.

Using western blot and SYBR Green Real time PCR we investigated 77 thyroid tissues including 19 goiter tissues, 11 follicular adenomas, 16 follicular carcinomas, 15 papillary thyroid carcinomas, and 16 undifferentiated thyroid carcinomas for total expression of PKM2, LDHA and FGFR1.

For this reason, sequences were aligned both to a reference transcript database (for estimation of expression of well-annotated transcripts) and to the human genome (for estimation of total expression of all exons, whether annotated or not).

We applied ASE to a set of genes (Table 1) with highly significant evidence for cis-regulation from total expression data [5], [7].

To analyze for membranous or total expression of FcεRI α-chain, cells were either permeabilized with 0.2% Triton-X-100 for 30 min, or left with PBS.

For most genes, total expression was associated with more than one reference signals.

(C ) Total expression for Angptl4 for embryos grouped by embryonic genotype and maternal genotype.