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Family consent for tissue use was obtained in collaboration with the provincially run Transplant Quebec organ donation program.
As discarded remnant tissue, it was exempted by the University of Rochester Institutional review board from patient consent forms beyond standard patient consent forms for tissue use.
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The brain regions were dissected, immediately frozen on dry ice, and stored at −80°C until use, except for tissue used ChIP assay samples, which was freshly processed.
Here, we investigate the ability to measure these parameters for tissue using spectroscopic optical coherence tomography (SOCT).
Consent for tumour tissue use was taken in accordance with national tumour banking procedures (UK: 05/MRE/04/70).
Approval from the Local Research Ethics Committee was granted for human tissue use, and procedures were in accordance with institutional guidelines.
Titer was calculated per gram of tissue and was compared between the wild type and Luc+ strain for each tissue using a t-test for unequal variances [40].
Lysis incubation was carried out at 65°C during 2 hr for cambium tissue and 1 hr for leaf tissue using CTAB 1% PVP buffer.
Spots of regulated metagenes are detected for each tissue using the 98% / 2% quantile criterion for over- / underexpressed metagenes, respectively.
This chapter outlines bioreactor design for soft tissue, using a bioprocessing approach.
Microarray data were GCRMA normalised independently for each tissue using GeneSpring GX (Agilent).
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