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Saeed and Nadia said this was impossible, and explained, in case he had misunderstood, that there was no problem, that they had paid the agent for three passages and would all be leaving together, and Saeed's father heard them out but would not be budged: they, he repeated, had to go, and he had to stay.
Mouse marrow MSCs were collected, cultured, and passaged for three passages and then identified by morphology.
After 2 weeks, the HAT medium was replaced with HT medium for three passages followed by returning to the normal cell culture medium.
In this study, we demonstrate that fibroblasts also maintain an epigenetic positional memory even after culture expansion for three passages.
Thus, fibroblasts and MSC maintain positional information in their DNA methylation profile even after culture expansion for three passages.
Competitions were repeated two to three times and each competition was continued for three passages by isolating virus at peak infection and subsequently infecting a fresh batch of host cells.
Similar(37)
Resistant cell lines were maintained in drug-free medium for three passage cultures before use.
hMSCs were treated with Ca2+/hypoxia for five passages in the absence or presence of U0126 or BAPTA-AM.
Sheep BMSCs and ADSCs were labeled with 2, 4 and 8 μmol of PKH26 and monitored for six passages.
After culture on primary ovine fetal turbinate (OFTu) cells for ten passages, the virus was named ORFV AH-F10 and maintained in liquid nitrogen for preservation.
50 mL cyanobacterial cell suspension containing PHB granules was homogenized using Panda Plus homogenizer (GEA Group AG, Düsseldorf, Germany) for ten passages at 1500 bar pressure at room temperature.
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