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All fluorescence images represent maximum intensity projections in two dimensions of three dimensional image data.
Performing this for each detected photon in a standard CSI scan would lead to three dimensional image formation.
19 Tumours were measured from two dimensional images at the maximal dimensions of the tumour.
For static imaging, three dimensional images were collected in a 176 x 176 x 112 matrix with a pixel size of 0.2 mm x 0.2 mm x 0.2 mm through a 40-min scan (a temporal resolution of 40 min), producing high resolution imaging data.
However, some distortion of the data and loss in resolution may occur in displaying the resulting three dimensional curving plane as two dimensional image (Fig. 4G).
Three dimensional images were then reconstructed from these two-dimensional gray-scale image slices using the reconstruction utility (GE Medical System) and visualized using Microview Software GE Medical SystemMicroview Software GE Medical System
Accelrys Discovery Studio Visualizer 2.5 [99] was used to produce three dimensional images of the models obtained.
Three dimensional images were rendered in Volocity (Improvision).
Three dimensional images were evaluated by epifluorescence microscopy including Apotome technique (Zeiss, Göttingen, Germany).
The specimens were cross sectioned and radiographed to provide three dimensional images.
For this purpose, two dimensional image arrays were constructed.
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