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PCR cycling was carried out with a 55°C annealing temperature for three cycles followed by 65°C for 37 cycles.
"I've been dreading this every election year for three cycles," said Jim Jordan, Mr. Kerry's former campaign manager.
In preparation for three cycles this spring, beginning next Saturday with the matinee broadcast of "Das Rheingold," the Met has already presented "Die Walkure" out of sequence.
The LP packet can hold the channel for three cycles.
Calli induced were subcultured for three cycles, on MSBM with same 2,4-D concentration.
(d) Reusability of Cu-ZnO catalyst for the degradation of Sudan red (III) for three cycles.
The mixture was degassed by vacuum/nitrogen purging for three cycles.
The regeneration of exhausted column bed was studied for three cycles of adsorption-desorption.
Moreover, the compounds were stable for three cycles of freezing and thawing.
Two horizontal wells conduct the steam stimulation in turn for three cycles according to the procedures above.
The column was regenerated using 0.1 M HCl solution and sorption desorption studies were carried out for three cycles.
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