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Blood and urine samples were first collected after 60 min and then samples were collected every 30 min for three consecutive time interval.
To evaluate the relationship between genome-wide expression profiles of the two genotypes in relation to the heat treatment, SAM multiclass analysis was performed for three consecutive time points, 0 h-2 h-6 h (Additional file 1, SAM multiclass).
The preliminary expression data were subjected to a filter for genes that exhibited at least a 1.15-fold differential expression ratio between induced and treated samples and mock-treated (-E2 -uninduced (+TET) controls at each time point and in the same direction for three consecutive time points or in four out of the five time points.
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This procedure was repeated for three consecutive times.
Routine urine for three consecutive times showed that all of the patients experienced microhematuria after biopsy, there were no patients with gross hematuria.
A choice was considered perseverative when an animal re-entered the same arm of the maze that it had visited previously for three consecutive times.
PSA progression was defined as the first day when the PSA was increased for three consecutive times or when clear clinical radiological evidence of progressive disease was seen.
The trapped Tenax beads were rinsed thoroughly with deionized water and were extracted by sonication using 3 ml of acetone-hexane mixture (1 1, v/v) in 20 ml scintillation glass vials for three consecutive times.
Test of tumor markers from the bile showed CEA of 66.89 ng/mL (0-5 ng/mL) and CA199 larger than 4000 U/mL (<37.0 U/mL), but examinations of exfoliated cells proved negative for three consecutive times.
Channel coefficients remain the same for two consecutive time intervals.
In this case, the busy period (B) is geometrically distributed, since the channel agreement probabilities for two consecutive time slots are independent of each other.
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CEO of Professional Science Editing for Scientists @ prosciediting.com