Exact(3)
The flasks were kept at 30°C with 150 rpm for 30 min for thorough mixing of the contents, followed by filtration through a wet muslin cloth with thorough squeezing, and the filtrate was centrifuged at 8,000 rpm for 30 min.
The mixture was left overnight on a mechanical shaker for thorough mixing.
The mixture was vortexed for thorough mixing and incubated on the cycler (72°C, 10 minutes) to enable 3´-A tail.
Similar(57)
If a new mutation appears, however, it will take quite a while for that thorough mixing to happen.
After thorough mixing for 10 s, samples were centrifuged for 10 min at room temperature at 20.800 g.
The addition of 1 mL of 3.6 M/L sodium thiosulfate with thorough mixing for 10 s stopped the oxidative process.
Multiplexed cocktails of biotinylated, reporter antibodies for each multiplex were then added robotically and after thorough mixing, were incubated for an additional hour at room temperature.
Multiplexed cocktails of biotinylated reporter antibodies for each multiplex were then added robotically, and after thorough mixing, were incubated for an additional hour at RT. Multiplexes were developed using an excess of streptavidin-phycoerythrin solution which was thoroughly mixed into each multiplex and incubated for 1 hour at RT.
To the complex mixture, 25 mL of 0.2 M Na2WO4·2H2O solution was added with constant stirring for an hour to achieve thorough mixing and complete precipitation.
1 ml of sterile water was added, and after thorough mixing centrifuged for 15 sec again.
Two milliliters of monoethanolamine buffer was added to 50 μL of the same sample used for OCN measurement, followed by thorough mixing.
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