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For this, samples were saturated under vacuum in a desiccator for 3 days.
There are several possible reasons for this: samples may have contained PCR inhibitors, DNA may have degraded during storage, or there may not have been any prey DNA present because the defecating bird had not fed recently.
For this samples were incubated for 30 min at 25°C with 4,000 U of λ-PPase (New England BioLabs) in reaction buffer (50 mM Tris-HCl pH7.5, 0.1 mM EDTA, 5 mM dithiothreitol, 0.01% Brij 35, and 2 mM MnCl2).
For this, samples were analyzed with a commercially available indirect immunofluorescent assay (Euroimmune, Lübeck, Germany) according to the directions of the manufacturer.
For this samples were collected at regular intervals, stained with the fluorescent dye Bodipy 493/503 and analysed by flow cytometry.
For this, samples were initially lyophilised and pulverized and an aliquot of the resulting powder was extracted with 80%% methanol in water [ 46].
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Results for this sample have a margin of sampling error of plus or minus three percentage points.
For this sample, the next sampling day was selected for pooling (Supplementary Table 4).
However, the authors reported a negative H1N1 qPCR result for this sample.
Apply Equation 20 to the sample training set in Figure 6.5 to estimate the best value of for this sample.
This sampling method requires special statistical analyses that account for this sample structure.
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