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For this assay, two cocktails were prepared as shown in Table 2, in two separate cuvettes and kept on ice.
For this assay, two argon laser lines were used.
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For this assay, three parallel competition populations were established with flies derived from three different populations, one from Okayama (population F1 in additional file 1), one from Tokyo (population F2), and one from the Ivory Coast (population F3).
For this PCR assay, two different pairs of oligonucleotide primers targeting the gene encoding for 85 KDa protein (common to all mycobacteria) and the insertion sequence IS6110 (specific for M. tuberculosis complex) were used [ 11– 11].
The total time required for performing this assay was two hours, compared to four hours required for LiPA v. 1 after PCR-amplification.
For each assay, two samples were counted for each treatment group, and the assay was repeated three times.
For ChIP assay, two controls were used: a positive and a negative control antibody.
For enzyme assay, two mL of freshly grown culture was taken and centrifuged at 10,000 rpm for 10 min.
For the reductase assay, two different types of reductase substrates were used.
For Matrigel plug assay, two weeks after subcutaneous injection, Matrigel plugs were harvested and histology performed.
For the EMSA assay, two probes were used: a wild type probe and a mutant probe.
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