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(A,B) Protocols and lentiviral reagents employed for this assay.
Plasma is not an acceptable sample for this assay.
We used a CYP24 promoter fused to a Luciferase reporter in HEK293T cells for this assay.
For this assay, ACTB, GAPDH, IPO8, RPL13A, SDHA, and TBP were used as housekeeping genes63.
Material from the 0 1-cm sediment interval was not available for this assay.
The reaction temperature and time for this assay were optimized.
Experimental conditions were carefully optimized for this assay strategy.
For this assay, ACTB, B2M, GAPDH, HPRT1, and RPL13A were provided on the array as housekeeping genes.
For this assay, we synthesized a reported small-molecular H2S donor22 that generates H2S by reacting with cysteine.
Data confirms that proteins were not filtered by μPAD, which is essential for this assay.
For this assay, primers and probes were designed for the kinetoplast minicircle DNA of L. infantum.
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