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To confirm the changes suggested by the microarray data for the ALAS1 and GATA3 mRNAs, we performed real-time RT-PCR on the same mRNA samples, using primers designed specifically for these transcripts [see "Real-time reverse transcription polymerase chain reaction (RT-PCR)"].
The wide-spread conservation of satellite transcription is consistent with a conserved regulatory role for these transcripts in gene regulation or chromatin modification [ 47].
The lack of defined function for these transcripts has led some to propose that they arise indirectly through spurious transcription of the genome.
For these transcripts, NMD serves as an important posttranscriptional regulator of transcript levels.
This suggests they should remain responsive in adult skeletal muscle, yet Myo-CELFΔ mice exhibit wild type splicing patterns for these transcripts.
The fold change values determined by qPCR are in good agreement with the microarray data shown in Table 3 for these transcripts.
Quantitative real-time PCR revealed no significant differences for these transcripts between mock and Mjd siRNA transfected C2C12 cells at Days 0 and 1 of differentiation (Figure S1).
Among their findings, they identified local eQTL for OCA2 and AKR7A2 in similar chromosomal positions to local eQTL for these transcripts that we identified in our study.
The observation of increased expression of RNAIII, hla and spa in MRSA252 in response to LC-uFFAs is significantly different to previously published experiments for these transcripts in alternative strains [34], [35].
Little is known or reported for these transcripts beyond their sequence, so much further work will be required before the significance of these relatively large transcriptional differences can be understood.
The expression levels for these transcripts were quantile normalized.
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