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An alternate method (' ad hoc' filtering, [ 20]) simply examines the replicates for their fold change between mutant and wild-type, as well as the coefficient of variation (CV) thereof.
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The elaborately wound turbans of Persia and India also have a skullcap as a foundation for their folds.
The formation of disulfides in proteins that enter the endoplasmic reticulum is essential for their folding.
However, many proteins need assistance for their folding in vivo (Hartl et al., 2011 ; Kopito, 2000 ).
In addition, larger proteins may be less stable and require more help from molecular chaperones for their folding.
This observation is perhaps because of the existence of several chloroplast proteins which require Cpn60 proteins for their folding.
Some proteins do not interact with GroEL, hence even though they are aggregation prone, cannot be assisted by GroEL for their folding.
These methods each had an average of 38% of features in common for their CV fold pairs when considering the top 50 features.
Mammalian cell lines should, in principle, be the best choice for the expression of human proteins with complex features that demand homologous post-translational systems for either their folding or modification (Braakman and Bulleid 2011).
The negative domain hits were then dissected into the fold-critical and remnant scores and then evaluated for their corresponding fold-critical and remnant E-values.
In the first phase of the study, only two potential biomarkers (miR-548q and miR-483-5p miR-483-5p miR-483-5plidation based on their fold change and P value.
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