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For the verification of the expression of the pca gene clusters, the transcripts of the pca genes in the strains under no H2O2-stress conditions were analyzed by RT-qPCR (Table 1).
For the verification of the expression of identified putative expansin genes in the rootstock Teleki 5C, a specific polymerase chain reaction (PCR) followed by sequencing (Agowa, Berlin, Germany) was performed.
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L.S. did the verification of the transgene expression and mainly performed the animal experiments.
Model checkers have been widely used for hardware verification, which allow the verification of the different properties in discrete systems.
Independent experimental verification of the expression levels obtained in our transcriptome and translatome experiments was performed for a set of differentially expressed T. cruzi genes.
Additionally, quantitative real-time PCR data provided an independent verification of the microarray expression values.
For verification of spatial expression, Cuscuta-RNA from the two tissue types was further characterized by Northern blot with a probe derived from the above said cDNA clone.
The verification of array results was based on their level of differential expression and the amount of annotation available for their corresponding BmiGI V2 sequence.
Based on common and specific biological processes revealed by RNA-Seq and proteomic analysis, we selected a subset of genes associated with metabolism, stress and environmental responses, for further verification of gene expression patterns by qPCR.
Following bioinformatics analysis, we collected goldfish brain samples at six seasonal time points for experimental verification of gene expression patterns.
These genes were selected for experimental verification of homoeolocus expression patterns.
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