The variation of quantification cycle (Cq) across samples was compared among three reference genes (B2M, Actb, Rpl10a) to select the most appropriate reference gene for our study.
The variation with the limits of agreement for repeat CT studies in emphysema is known [ 8, 9]; however, to our knowledge no information is available on the variation of CT air trapping quantification.
(2) Plot of the variation of the centre of mass due to kinetics and motion (crude motion detection and quantification).
A magnitude of 20% was selected, because the FDA has set the limit of precision in analytical method to 15% coefficient of variation, allowing a 20% coefficient of variation for the lower limit of quantification.
In addition to reducing the variation in quantification of uptake for individual hotspots across different reconstruction protocols, SUVref also significantly reduces the variation in assessments of change in uptake when both the baseline and follow-up scans are reconstructed using the same protocol.
A range of the values for the limit of quantification (LOQ) was between 1 and 10 ng/ml and coefficient of variation (CV) of 3-15% was estimated [123].
Modelling fMRI data using parametric regressors allows for the simultaneous quantification of variation in many stimulus dimensions and may be very useful for simultaneously isolating and statistically assessing the contribution of variation in different dimensions.
To correct for variation associated with quantification of amplicon target (quantification variation), and downstream variation associated with target fragmentation, labeling, hybridization, washing, staining and scanning (microarray technical variation) a two-step normalization procedure was developed.
However, the lack of an established and generally accepted marker combination and of a standardised protocol with regards to reagents and gating strategies may account for the widespread interlaboratory variations in quantification of EPC [12].
Percentage coefficient of variation at the lower limit of quantification (LLOQ) varied from 2.26 14.69.
