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She developed the quantification procedure and carried out the quantitative analysis with angiogenesis inhibitors for the validation of the assay.
These cDNAs were kept in aliquots at -20°C and used for the validation of the assay, prior to the analysis of patient's samples.
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A cross-validation of the DBS assay was not possible because no HPLC-UV assay applying DBS samples was available, but for the validation of the plasma assay, an accredited HPLC-UV was used.
Three discrete sets of clinical samples (totalling 655 cancers and 655 normals) were used for clinical validation of the assay.
A pilot screening provides further evidence for validation of the assay.
This paper provides recommendations on best practices for the validation of cell-based NAb assay and suggested validation parameters based on the experience of the authors.
MA performed the experiments for the initial characterization and validation of the assay procedure.
We present clinical validation of the assay and a detailed framework for design and validation of similar clinical assays.
The validation of these assays therefore merits urgent investigation.
Additionally, the ELISA assay enables detection of a single antigen, a drawback for the validation of biomarker panels.
A total number of 100 clinical specimens were tested for validation of the molecular assay.
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