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Combined with computational prediction, simultaneous profiling of miRNA and mRNA expression levels may be a strategy for the validation and identification of functional miRNA targets [31], [32].
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A set of 44 SNPs were selected for further validation and identification of alien addition and translocation lines.
A database of surgical patients held by the local tertiary care centre (East Midlands Congenital Heart Centre, EMCHC) was used for validation and identification of additional cases.
For validation, independent coding and identification of themes were conducted by five investigators.
The validation results revealed that the developed method is highly efficient and reliable, and hence suitable for quantitative analysis and identification of FC samples.
Other possible uses include the validation of multi-gene signatures, and identification of novel gene signatures and biomarkers for patient survival and response to chemotherapy.
We entered data into dBase IV and used Microsoft Access 10 for cleaning and validation, including identification of duplicates and audit for transcription errors.
Criteria for validation included identification of at least 3 peptides per protein and a probability of protein and peptide identification above 95%.
Mascot Percolator [100] is a Java library and tool designed for the validation of Mascot identified peptide/protein identifications.
In this report, we detail the different approaches used for the isolation, the identification of a RNA binding protein and the validation of its role.
The r-values, which are not directly used in the identification procedure, served for the validation of the models and to demonstrate their improved accuracy.
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