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Herein, the minor variations/differences with regard to the respective diagnostic peaks allow for the unambiguous annotation.
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Their large size enabled the unambiguous annotation of 3,858 (22%) UniTags to genes or proteins in public data bases and thus to stress-response processes.
In 2004, two genome sequencing projects for B. mori were reported independently by Chinese and Japanese teams; however, the datasets were insufficient for building long genomic scaffolds which are essential for unambiguous annotation of the genome.
Sequencing reads from one arm of the RNA hairpin usually greatly exceeded those from the opposite arm, enabling unambiguous annotation of the duplex miRNA and the miRNA*.
However, only a reduced number of tags matched more than one location in the genome (28 tags, 4%), suggesting unambiguous annotation for most 26-bp tags.
The list is based on Additional file 3: Tables S3 and Additional file 4: Table S4 for genes with unambiguous annotations.
Still, some sequences have been observed for which the application of these rules did not result in a single unambiguous annotation, but instead allowed for multiple plausible alignments (haplotypes).
Because these genes had relatively unambiguous annotation, they were selected for tea-specific pathway analyses.
This approach led to unambiguous annotation of 10'800 transcripts corresponding to 57.4percentnt of the contigs assembled from the normalized 454 long-read library.
Gene names allow for the unambiguous identification of the gene.
For the unambiguous loci, the two methods are exactly the same.
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