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For the third set of samples the final residue after evaporation of the solvent was left dry for 30 min before re-dissolving.
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The repeated-sampling-approach requires two sets of samples and uses point-specific values, the 137Cs inventories of the first set of samples, as the reference 137Cs levels.
For the first set of three samples the usual procedure was followed whereas for the second set of samples the SPE column was run dry for 10 min after application of the aqueous extract and before the wash step.
For the first set of samples (samples 1 33, see Table 2) faeces were collected on a Thursday and sent by ordinary regular mail without a cooling device.
Quite similar expression levels were seen in the second set of samples for Robusta grain germination (FRT05; data not shown).
The first set of samples was taken from prepared test solutions for concentration verification.
The reproducibility of this data was high with the second set of samples being predictive of the first set.
As a result, previously characterized mutations from all control samples were identified in the first set of samples, regardless of the size of the pool.
The first set of samples are those with resonance frequency higher than nominal values and the second set includes samples with resonance frequencies lower than the nominal value.
The second set of samples will be the trial blood samples.
A third set of samples was collected and kept unfiltered and non-acidified in glass bottles for bacteriological counts.
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