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Here, we describe a simple method for the synthesis and purification of POCs.
To address these limitations, we have optimized known methods for the synthesis and purification of linear, K11-, K48-, and K63-linked ubiquitin dimers, trimers, and tetramers on a preparative scale.
The authors wish to thank Luis Rodrigues and Florela Penea for the synthesis and purification of peptides and Thomas Smith for discussion and all of the blood donors.
The wild-type double-strand cDNA synthesis kit (Affymetrix) was used for the synthesis and purification of cDNA, and a Nanodrop spectrophotometer was used for cDNA quantification.
Translation extracts were supplemented with increasing amounts of Hbs1-DN, and used for the synthesis and purification of scanning and engaged RNC complexes via the nascent chain.
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At 8 and 24 weeks diabetes, the synthesis and purification of methylglyoxal (MG), as well as the synthesis of the derivatising agent and the standards for determination of MG, were prepared according to published procedures [ 14].
The synthesis and purification of 1 was performed as previously described.
The synthesis and purification of ProGlo was performed as previously described.
Ultrasensitive determination of the chiral nature of a molecule is important for fully understanding protein and DNA structures, and for the design, synthesis and purification of drug molecules.
A generic protocol for the automated synthesis and purification of MIP nanoparticles has been developed and described earlier [5].
We have previously published detailed methods for the design, synthesis, and purification of KOD.
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