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This pre-test aims at verifying the feasibility of the methods to be used for the subsequent testing of the full-scaled BU mock-up.
During the standardization of the technique, the densitometric values observed for positive controls (larvae treated with 50% methanol, a concentration known to kill the larvae [ 25, 26]) were indeed proportional to the number of dead larvae, demonstrating the ideal number of larvae for the subsequent testing trials.
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In other words, taking the test had a washback effect on their preparation for the subsequent test.
As a result, 1 ng/μL was adopted as the optimized concentration of the substrate peptide for the subsequent test (Fig. 3).
Refreshing trials with the reinforced CS+ and CS− were intermingled between the tests to ensure motivation for the subsequent tests.
The offspring were grouped, 11 12 for each, and used for the subsequent test.
The supernatant was used as stock solution for the subsequent test.
The dosage of 10 mg/kg was chosen for the subsequent tests based on other reports.
These baseline measures of activity were used to match activity levels across the injection or infusion group allocations for the subsequent tests of the locomotor effects of SKF81297.
The main advantage of the feature elimination is to avoid overfitting, which means a perfect fit of the decision function for the training dataset, but a failed fit for the subsequent test datasets.
For the initial validation set of B-CLL samples (n = 4, σ = 0.3) the delta threshold was calculated to be 0.5, for the subsequent test set (n = 10, σ = 0.3) the delta threshold was calculated to be 0.3.
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