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Test samples for the study were generated by diluting the inactivated virus stock solutions in human fresh-frozen plasma testing negative for HIV-1, hepatitis B virus, hepatitis C virus, and SARS-CoV by RT-PCR.
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However, animals for the longevity study were generated by crossing heterozygous animals in order to obtain sibling controls.
The randomization schedules for each study were generated by GlaxoSmithKline Vaccines using validated software.
For the Rb studies, myocardial images were generated by averaging the images from 90 600 s.
The probes used for hybridization were generated by PCR.
Figures for structural snapshots were generated by using Pymol.
Chimeras for Hox genes were generated by fusion PCR.
Templates for riboprobe production were generated by PCR.
Probes for Foxl2 and Gata4 were generated by RT-PCR.
p values were generated by Bonferroni correction for multiple tests.
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