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The sample for the study was calculated using data from our recent pilot study [ 36].
The sample size for the study was calculated using one-stage binomial design.
The sample size for the study was calculated using RAAB software [ 7].
The Complete Bouguer Anomaly values for each gravity station used in this study were calculated using a density of 2.5 g/cm3.
The power for our genetic association study was calculated using the Power for Genetic Association Analyses PGAA) [ 25].
Interrelation between parameters studied was calculated using the Spearman correlation.
Next, correlations among study variables were calculated using Pearson's correlation coefficients, providing initial support for the hypotheses.
Pair-wise correlation coefficients for gene expression among the studied genes were calculated using R.
Costs for the 1-year study period were calculated using the 3-, 6-, 9- and 12-month follow-up.
Except for optimal contributions, which were calculated using Fortran, all measures were calculated using Visual Basic.
For the validation studies, HWE tests in controls were calculated using PLINK 1.07 software.
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