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A rapid and simple method for the separation and quantitation of gentamicin sulfate by HPLC coupled with evaporative light scattering detection (ELSD) has been developed.
A reversed-phase liquid chromatography (LC) method has been developed, optimised and validated for the separation and quantitation of capsaicin (CP) and dihydrocapsaicin (DHCP) in a topical cream formulation.
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The separation and quantitation of the enantiomers and also the determination of the enantiomeric purity are now current and indispensable tasks for the pharmaceutical analysis.
In literature, only one report has been found describing the separation and quantitation of process-related substances to VRC tartarate [13], however there was no report on the stability-indicating assay for VRC.
The conditions allow the separation and quantitation of CP and DHCP without interference from the other substances contained in the cream.
The developed HPTLC method is the first one reported for separation and quantitation of Flutamide and its metabolites, while the HPLC DAD method has high selectivity, precision, and short analysis time (< 10 min).
The addition of a third dimension to the previously reported "coupled-column" systems, and further modifications made, has allowed for optimal identification, separation, and quantitation of micro-components in complex mixtures.
We developed two independent approaches for separation and quantitation of 24 oxygenated polycyclic aromatic hydrocarbons (OPAHs) using both liquid chromatography-atmospheric pressure chemical ionization/mass spectrometry (LC-APCI/MS) and gas chromatography-electron impact/mass spectrometry (GC-EI/MS).
This is likely due to the cumbersome and lengthy tasks associated with the individual extraction,, separation and quantitation of lipids and their corresponding subclasses.
A method for fast chiral separation of cetirizine and quantitation of levocetirizine in human plasma using subcritical fluid chromatography with tandem mass spectrometry was developed and validated.
The remaining cells were used for lipid isolation and quantitation of cholesterol concentration using the colorometric Cholesterol/Cholesteryl Ester Quantitation Kit (Calbiochem).
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