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Since the two thinned and un-thinned pole stage stands, respectively were pooled for the selection of sample tress we finally had 162 sample trees.
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Our concept for an integrated workflow for sample data spans from the selection of sampled individuals to the aggregation of character data for named taxa, but intentionally it excludes the capacity to conduct evolutionary analysis of sampled individuals.
For the selection of samples in our study, several sample folds with their ages and residential histories were collected to determine whether they were native residents.
The criteria for the selection of samples for this study were that the proportion of both IUGR- and LBW-affected newborns would be 10 15%.
Important information is often absent, including crucial aspects of sample collection and preservation, for example, the selection of sampling sites, exposure, sampling period, age and condition of target compartments.
For comparison reasons, the selection of samples ensured considerable differences in their physical properties.
For this reason, the selection of samples is very important to provide the largest range of information for the building of the calibration data set [ 14].
The decision to take samples for microbiological culture and the selection of samples was made by the physicians.
Initially, the selection of samples for genotyping was based only on breed, without regard to disease phenotype.
The selection of samples for molecular typing using MLVA was based on qPCR assays that have been used for the detection of C. burnetii in several studies [ 5, 16, 17].
The selection of samples for further PCR assays aimed to include cultures representative of most clinically relevant species of Candida based on the initial phenotypic identification obtained in our clinical laboratory of the yeasts growing in blood cultures.
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