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Ethical approval was not required for the secondary analysis of this anonymised data source.
Current mood symptoms were determined from patient report, as assessed by the STEP-BD Affective Disorders Evaluation at the time of enrollment, and clinician observation and reflected any mood symptoms in the 10 days prior to enrollment for the primary analysis, and mood symptoms thresholded for occurring on at least four or seven of the 10 days prior to enrollment for the secondary analysis.
For the secondary analysis, malaria incidence was estimated for the treatment and control groups.
For the secondary analysis, severe malaria cases were grouped according to their criteria for malaria severity, and compared separately to matched controls.
In order to examine our data for the secondary analysis, where random wells were assigned to standards and unknowns, we used a classical ANOVA model to explain the variation in the log-transformed data in terms of five factors (well, plate, duplicate, sample type and subject) and pairwise interactions within all the factors with exception of the well variable.
For the secondary analysis, osteoporosis status was indicated by survey participant reporting during the NHANES interview.
We also re-run our primary analysis using the same data as for the secondary analysis.
Ethical approval for the secondary analysis was gained from Cardiff University.
For the secondary analysis we will develop a propensity score model as published before [ 43, 44].
All forest plots for the secondary analysis are provided in the supplementary files.
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KR conceived the research concept, secured funding for conducting the secondary analysis, verified the analysis outcomes, and helped draft the manuscript.
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