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In the middle panel, the other two genes of this group (AT5g60250 and AT1g65430) are singled out to show that they have a particularly high level of expression in just one developmental sample, which is again mature pollen (8 times the average of the rest of samples for AT5g60250 and 23 times for AT1g65430).

For HR E1.5, the ratio was slightly lower, 0.24 for sampling point 1 than for the rest of sampling points (0.25-0.27) but no statistical difference was found.

To this extent, the ANNs are trained with a few samples, following a conventional MCS procedure and used henceforth to predict the stochastic response for the rest of samples.

Except one control plasma sample which were excluded from the analyses, the CVs of replicates for the rest of samples were under 13%, which were below the manufacturer's recommended 15% standard.

It was also observed that the low expressed genes had a mean level of expression in mature pollen (mean: 52.5±9.5) that was in average about 5 times higher than the mean for the rest of samples (10.7±1.0).

For Synbreed samples the length of paired reads was 76 bases while for the rest of samples it was 101 bases.

conditioned to the rest of sampled variables.

Tubes containing S-Wet samples (1 ml) were also vortexed for 3×15 sec and then, 0.5 ml of each sample was used for nucleic acid extraction and the rest of sample was frozen at −20°C for storage.

The rest of samples was heated statically.

For the rest of the sample considered the "observed" sample, non-anchor SNPs were masked, but retained and considered to be the "true" genotypes.

Convenience sampling 34 was suitable for the rest of the sample.

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