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The absence or presence of Sr2+ at up to 0.1 mM in the reactor solution and up to 0.017 M in the titrant solution appeared to have no effect on the rate of calcite precipitation that could be statistically distinguished from the standard deviations calculated for the replicate experiments.
RNA for the replicate cell lines was from different biological samples.
The R2 for the replicate microarray chips was found to be R2>0.98, which indicated negligible total variability between replicates.
Positive selection was claimed to be detected for the replicate data set if the LRT was significant in at least one window.
In each replicate, we permute the case/control status and perform the FET on the permuted sample, from which the provisional index for the replicate is obtained.
For the replicate sets, the maximum calculated Z' values were 0.76, 0.91, and 0.59 for FLIN, NVAR, and CORR2 and ranged between 0.46 and 0.91 depending on groups and measurements being compared (Supplementary Table S3).
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Values are mean ± standard error for the replicates.
One pot was kept as stock for the replicates.
Next, the average percentrank and the average weighted percentrank were identified for the replicates of each sample.
Error bars show the SEM for the replicates.
Three biological replicates were performed and the average YFP intensity for the replicates is reported ± SE.
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