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RPKM of each gene was calculated based on the length of the gene and reads count mapped to this gene, considering the effect of sequencing depth and gene length for the reads count at the same time.
The best matches in the genome were used as the location for the reads with multiple matches.
The MIRA assembler used for the reads assembly identified a total of 31,000 possible SNPs over 4.579 Mb of sequence.
The matching quality scores for the reads were also removed.
The length distribution for the reads is shown in Figure 1A.
On one hand, they can help disambiguating the isoform of origin for the reads.
This estimation is done separately for the reads aligned in forward and reverse direction.
For the reads that passed the quality filtering step, mapping was done using NOVOALIGN [ 59].
Dyad positioning scores were independently calculated for the reads obtained from the + and - strands.
Modeling insert sizes contributes to increased estimation accuracy by disambiguating the transcript of origin for the reads.
The average Phred quality score for the reads was 37 ± 0.05, with a read length of 51 bp.
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