Standard curves for each gene were performed using the undiluted and diluted cDNA to cover the range of all template concentrations.
Standard curves for each gene was performed using the original and diluted cDNAs to cover the range of all template concentrations.
For Figures 1 and 2, the linear range of template for multiplex polymerase chain reaction (PCR) was determined empirically and PCR-amplified products were quantitatively measured using Labworks (UVP Inc., Upland, CA, USA) as described previously [ 1].
The present study has reported a novel method for DNA fragmentation using the MspJI enzyme, which has been exemplified for a range of template types.
As illustrated in Figure 3B, bands of the expected sizes, and the expected sizes only, were easily detected in all reaction lanes, demonstrating tolerance of the assay for a wide range of template concentrations.
For such samples, the same dCt values were yielded for a broad range of template input, from 150 ng/reaction down to 1.5 ng/reaction (Figure 3A).
BIOTAQ polymerase is a basic Taq that can be used for a wide range of templates, whereas Diamond polymerase has been modified by a point mutation at the active site of the enzyme, enabling it to read through regions of secondary structure, microsatellites, and guanine cytosine rich templates, such as those found in the M. tuberculosis genome.
Beyoncé even cites Carey's runs on "Vision of Love" as the template she followed for experimenting with the range of her own voice.
For relative quantification in qPCR, a mathematical model was used that incorporated the effects of the efficiency of amplification for each primer pair over a 10 range of template dilutions and starting template concentrations were normalized by comparing to β-actin amplification.
