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For the purpose of normalization, visceral adipose validated housekeeping gene ACTB was used as the reference [ 24].
For the purpose of normalization, the membranes were stripped, and re-probed with antibodies against β-actin (1 10,000; Sigma, St Louis, MO, USA).
Ct values of target genes greater than 37 were considered to be a negative call and assigned a value = 37 for the purpose of normalization.
Despite the differences in algorithms and assumptions, both statistical methods were in agreement on the identity of the twenty most stable genes (Table 1), most of which are novel for the purpose of normalization studies.
However, levels of U6 (also called RNU6-1), whish is commonly used for the purpose of normalization between samples, and U6/SNORD44 ratio were elevated in the sera of breast cancer patients who did not have active disease.
For the purpose of normalization, a figure of 100%, corresponding to the maximum peak during the Dubowitz maneuver, was taken as a point of reference and used to normalize every signal.
Similar(53)
The purpose of normalization is to correct for non-specific variation, such as differences in RNA quantity and quality, which can affect efficiencies of the RT and PCR reactions.
The purpose of normalization is to correct for variability associated with the various steps of the experimental procedure, such as differences in initial sample amount, RNA recovery, RNA integrity, efficiency of cDNA synthesis, and differences in the overall transcriptional activity of the tissues or cells analyzed.
The purpose of normalization is to reduce the effect of light condition for the definition evaluation.
Negative and positive controls (vehicle, 1 µM NPY) were included on every plate for the purposes of normalization; for mutational studies, these controls were provided by the dual cell line expressing the relevant native Y receptor.
For the purpose of data normalization, the non-targeting RISC-free transfection was used as negative control, while ubiquitin B (UBB) and polo-like kinase 1 (PLK1) served as positive killing control.
More suggestions(16)
for the lack of normalization
for the denominator of normalization
for the normalization of normalization
for the calculation of normalization
for the effect of normalization
for the construction of normalization
for the estimation of normalization
for the purpose of standardising
for the purpose of normalisation
for the purpose of standardizing
for the purpose of normalization
for the purpose of advocacy
for the purpose of survival
for the purpose of contraception
for the purpose of photosynthing
for the purpose of blackmail
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