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Looking back to as early as the mid-1980's, rice geneticists and molecular biologists have always been interested in studying the architecture of promoters, initially for the primary objective of discovering regulatory sequences for spatio-temporal engineering of transgenes.
We chose length of ICU stay as a clinically important outcome for the primary objective as it was one of the key variables found to be significantly associated with vitamin D deficiency in the largest prospective study of 326 children published till date [4].
The main analysis for the primary objective was based on the number of hours between infectious challenge and blood stage parasitaemia.
The sample size was calculated for the primary objective taking the prevalence to be estimated of 50% that gives the maximum sample size, with 95% level of confidence and 5% bound on the error of estimation.
For the primary objective, the study had a 90% power to detect a difference between the proportion of subjects experiencing a grade 3 solicited or unsolicited general reaction following vaccination, should the difference in the proportion afflicted exceed approximately 20% to 30%, depending on the rates in the control group (rates of between 5% and 50% were assumed).
The calculated power for the primary objective was 78%%.
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Formal sample size calculations were not applicable for assessment of the primary objective (safety/tolerability profile).
The evaluable immunogenicity population for analysis of the primary objective included 207 patients (155 adults and 52 children); the most common reason for exclusion from this population was receipt of <3 doses of PCV13 in the sequence assigned.
The Reference Standard is the method that was prospectively identified in the clinical protocol as the method that would be used for the analysis of the primary objective.
In the pretreatment of feeds for catalytic cracking and for HDA, the primary objective is to reduce the amount of organic sulfur and nitrogen compounds in the feedstock [Catal. Rev.-Sci. Eng. 36 (1994) 75] [1].
The immediate response to this outcome, which would ordinarily be viewed by any rational actor as good news, is revealing for how it identifies the parties such as Netanyahu for whom the primary objective in shaping policy towards Iran is not to find a mutually acceptable resolution, but to engineer a war.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com