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For the preparation of sample A, a conventional cleaning procedure using acetone and methanol was performed.
The procedure used for the preparation of sample solutions was significantly simpler than that proposed by other authors and basically consisted of dissolving in water.
For the preparation of sample for TEM analysis, the beads were ground into powders and dispersed in ethanol, and then the suspension was sonicated for 10 min.
For the preparation of sample used for high spatial resolution analysis, the concretions were cut in half by a diamond wafering blade cutter (102 × 0.3 × 12.7 mm; Buehler, LTD., USA).
Because of adventitious properties of PVP CMC hydrogel like: porous internal morphology, quite a good moisture/solvent/solution absorption capacity, flexible in nature for the preparation of sample for testing in different shape, size and thickness, this bio-based hydrogel has been chosen to be used as a matrix for the preparation of calcite (CaCO3) incorporated hydrogel.
The analysis of reported results suggests that the procedure adopted for the preparation of sample C2 (Table 3) produced a catalyst characterized by satisfactory values of immobilization yield, for example, satisfactory rate of decolourisation, and low adsorption capacity of RBBR.
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Ultrapure water was prepared by a Milli-Q50 SP Reagent Water System Millipore Corporationn, MA, USA) for the preparation of samples and buffer solutions.
For the preparation of samples 'c' and 'd', Pt acac 2 and FeCl2 were used, respectively (Table 1).
For the preparation of samples to analyze mineral composition, wet digestion procedure was used.
For the preparation of samples for TEM, the NP solution was drop-coated onto the carbon-coated copper grids of size 400 mesh.
The procedures utilized for the preparation of samples for 2-DE are critical to the acquisition of high-quality results for the discovery of biomarkers.
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