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For this, cells were incubated with QDs for the predetermined time intervals and then washed to remove any unbound QDs.
Followed by 24 h preincubation with the first dose of IL−1β, the constructs were then given ultrasonic stimulation (frequency 1.5 MHz and SATA 30 mW/cm2) once a day up to 5 d for the predetermined time.
However, there were no significant differences in cell proliferation between the MG-63 cells cultivated on PCL and on AAc-PCL for the predetermined time intervals (∗ P < 0.05).
The antibiotic in each specimen was allowed to elute in buffered saline (PBS) in a shaker for the predetermined time periods of 1, 3, and 21 days.
b Relative OsUbc13 protein level in rice seedlings after treatment with various stresses, two weeks after germination rice seedlings were treated with the given biotic or abiotic agent for the predetermined time as indicated on top of each panel and total proteins were extracted from the whole plants.
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The circuit's organizers, however, stopped work on Nov. 15 after not receiving a contract from Formula One boss Bernie Ecclestone within the predetermined time frame for the November race, which was to be called the United States Grand Prix.
At the predetermined time point, an aliquot of 20 μl volumes were removed from each test suspension and plated on SDA for determining the colony forming units.
Finally, the Erlenmeyer flasks were placed in the oven for predetermined time at a constant temperature (details in Table 1).
Within the treatment groups, every patient received fixed doses for a predetermined time despite the individual phenotype of asthma.
Purchasing a put option on a stock you already own (commonly called a protective put) gives you the right, but not the obligation, to sell your shares at a specified price (the strike price) for a predetermined time.
Then, a specified mass of nano-LPMO powder (adsorbent dosage) was added to 10 mL of the Eosin aqueous solution, and the achieved suspension was immediately stirred for a predetermined time.
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