We examined the phosphorylation site localization of the peptides that were validated by Debunker.
During mitosis, phosphorylation and cytoplasmic localization of EBP50 was detected.
Our methodology, described in this article, incorporates a broader range of spectral features and seeks to identify evidence for the specific localization of the putative phosphorylation sites.
The PBD domain is responsible for the proper cellular localization of Plk1 through an array of phosphorylation-dependent protein protein interactions mediated by the phosphopeptide-binding groove.
These observations suggest that phosphorylation of FtsZ is required for the interaction between FtsZ and FtsQ as well as for the septal localization of FtsQ.
GCP-WD phosphorylation promotes interaction with the augmin complex, which was recently shown to be required for the spindle localization of the γTuRC [15] [18].
These results argue against the role of FRQ phosphorylation in the cellular localization of FRQ.
ID also inhibited the phosphorylation of IκBα and the nuclear localization of NF-κB (Fig. 4f).
Cytosolic changes in [Ca2+]i are also required for optimal TLR4 trafficking to the endosomes and the phosphorylation/nuclear localization of IRF3 [ 44].
This is supported by previous reports that phosphorylation impacts the subcellular localization of hnRNP I. Protein kinase A (PKA) serves a kinase for hnRNP I phosphorylation.
