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The letters GATC stand for the nucleotide bases guanine, adenine, thymine and cytosine, which are read by the cell when genes are active.
Positive CHIKV samples were processed for the nucleotide sequence of E1 gene region.
For the nucleotide positions where array calls were made, false negative rates were 1.41% for heterozygous mutations.
The average depth of read coverage for the nucleotide differences identified using the dog reference assembly and applied in subsequent analyses were 20.0 for the giraffe from MA1, 21.6 for the Nashville Zoo (NZOO) giraffe and 16.8 for the okapi.
A sequence homology search with the BLAST program showed that it had the highest homology (96.8% for the nucleotide sequences) to human chromosome 18pter-p11.23 clone 34 mRNA (Yoshikawa et al, 1997), a human EST.
The generated information is deciphered not only for the nucleotide sequence of the corresponding genome, but for its functional genomic expression at different conditions and states.
The P-loop is responsible for the nucleotide binding.
References for the nucleotide sequences used in this work are given in Table 1.
For the nucleotide site that normally binds ADP very tightly, NaDodSO4/polyacrylamide gel electrophoresis after photolysis indicates that primarily the beta polypeptide chain is appreciably labeled (86%), although some labeling of the alpha polypeptide chain is found (14%).
The data reported in this work for the nucleotide sequence of the 16S rRNA (1089 bp) gene of the isolate SJ3 have been deposited in the DDBJ/EMBL/GenBank databases under Accession Number KT222887.
ChromasPro (v.1.33) and sCAP3 software packages were used for consensus alignment for the nucleotide sequences.
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