RNU6B (ID 001093) was used for the normalization of miRNA quantification.
A second fluorophore conjugated to an antibody is used to detect the epitope tag C-terminal to the scFv, which allows for the normalization of expression and eliminates nondisplaying yeast from quantification.
RefGenes therefore allows to select experimental conditions that are similar to that of a specific experiment and to obtain reliable and condition-specific candidates for the normalization of RT-qPCR or other types of transcript quantification data.
Anatomical normalization parameters were used for the normalization of functional volumes.
A critical factor for creating reliable data in relative quantification is the normalization of the expression data of genes of interest.
It enables the normalization of deviance.
This method could be particularly useful for quantification of miRNAs in those biological samples, such as plasma/serum or exosomes, where lack of consensus for the normalization strategy prevents clinical applications.
Protein quantification of FAIM-L using the neuronal marker NeuN for the normalization revealed similar trend.
The procedures used for the quantification and normalization of gene expression levels are described in the Supporting Online Materials [ 40].
TBP was used as a housekeeping gene for quantity normalization; relative quantification of gene expression was calculated according to the method of 2−ΔΔCt described in the User Bulletin no. 2 and the Relative Quantification software version 1.3 of Applied Biosystems.
BjACTIN2 was used as a normalization control for the relative quantification of transcript levels.
