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Established reverse transcription PCRs for the medium segment were unsuccessful.
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Our data showed 99%100%% identity for a fragment of 913 bp of the medium segment, supporting identity using different sequences.
The largest RNA segment, L, encodes the L protein, the medium segment, M, the glycoproteins, and the smallest segment, S, the N protein.
The sequences obtained for the 2 medium segments encoding the ANDV glycoproteins Gn and Gc were compared separately (data not shown) and concatenated.
The LACV medium segment was used to infer phylogeny.
First, all strains exhibited some genetic change in the same medium segment untranslated region.
The virus genome contains 3 segments of negative-stranded RNA; the large (L) segment encodes viral RNA-dependent RNA polymerase, the medium (M) segment encodes glycoprotein precursor, and the small (S) segment encodes nucleocapsid protein (4 ).
The virus genome consists of 3 segments of negative-stranded RNA; the large (L) segment encodes viral RNA-dependent RNA polymerase, the medium (M) segment encodes glycoprotein precursor, and the small (S) segment encodes nucleocapsid protein.
RVFV RNA was identified in samples by using the specific one-step reverse transcription PCR (RT-PCR) described by Battles and Dalrymple (12 ), which is specific for 369-nt region of the medium (M) segment of RVFV RNA.
All mutations occurred in the small or medium segments: no polymorphisms were detected in the large segment.
This was perfect for the medium.
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