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Therefore, we used LPS as a stimulator to mimic the inflammatory environment for the later experiments.
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So the treatment duration for most of the later experiments was day 3-8.
However, an MDR suspension cell line and its corresponding sensitive line, were used for the later experiment due to the ease of maintaining and handling suspension cell line over that of adherent cell line which need constant trypsinization.
Because the formation of glucose laurate reached a plateau after reaction for 5 h, for later experiments conversions obtained at 5 h were reported.
They would also lay the foundation for his later experiments in architecture and exhibition design.
In this first experiment the GTP was added immediately after mixing BtubA and BtubB, while for later experiments the mixed subunits were allowed to incubate for two min before adding GTP.
For convenience, pulse mode sonication was adopted as the standard procedure for later experiments.
As control animals were destined for other later experiments, they were not equipped with invasive catheters.
The inhibitory effect of ICI182780 was observed similarly at concentrations from 10 nM to 50 nM, therefore 10 nM was selected for our later experiments.
For later experiments we will consider the use of immunohistochemistry, electron microscopy or PCR in the detection of bone.
Pseudo-viral particles generated by 293 FT cells within 48 hours were centrifuged at 100,000 g for two hours and frozen at -70°C for later experiments.
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