For all the acceptance that marijuana has gained in recent years – four states allow the sale of pot for recreational use, and 23 states and Washington, DC allow marijuana for medical use – the lack of normalization among marijuana sales may slow the process, since it gives opponents an opening to criticize.
Despite this, it is important to note that the lack of normalization approaches for RNA-Seq, makes identification of rare, cell-type specific transcripts challenging, as highly expressed transcripts would obtain the most tag coverage.
However, the lack of normalization of the information handled in and exchanged between such services hinders the scalability and extendibility.
Because there are no genes with differential expression in any of the within-condition normalizations, the lack-of-variation assumption only affects the final between-condition normalization.
The lack-of-variation assumption underlying current methods of normalization was self-fulfilling, removing variation in gene expression that was actually present.
Then-Brazil Ambassador Celso Amorim, (now Defense Minister & formerly Foreign Minister) defined the lack of progress in the normalization of peace as embedded in the region's proclivity for conflict.
Given the lack of gold standard for EMG normalization, while it has its limitations and must be interpreted within these limitations, the MVIC is still the best standard for comparison among muscles [ 25, 45].
This lack of normalization and centralization hinders the large-scale integration of brain connectivity results.
This explanantion was recently challenged based on the lack of statistic power and the oversimplification of normalization method [39], [40].
However, the existing normalization methods pose limitations due to the lack of good models to account for sources of experimental and biological variations [ 24].
