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In some cases, the unlabelled cell fraction (flow-through after positive selection) was recovered and used for the isolation of an additional cell type.
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Segregation analysis on a selective medium allowed for the isolation of single-locus, homozygous insertion lines (see additional file 2 for segregation results).
Improvements could be gained through selective breeding and the isolation of additional species and populations (Anbesse et al. 2013).
(PAO1 does not produce ExoU, which avoided the isolation of additional anti-ExoU hybridomas).
An additional six rats were used for the isolation of peripheral blood mononuclear cells.
Additional file 1. Overview of the PARE-seq method for the isolation of uncapped mRNA.
In addition, the cell lysis and tissue processing solutions used for the isolation of DNA should be examined for their contribution to excessive chemical noise, and the DNA isolation process could be modified by including additional DNA purification after isolation.
Finally, advances in techniques for the isolation of single cells are on their way.
The PolyATract mRNA Isolation System III (Promega) was used for the isolation of 300 ng mRNA.
Details of the isolation of total and poly(A)+ RNA are described in Additional File 6.
Although the isolation of either agent was sufficient to classify a farm as infected, additional criteria were required for those cases where isolation was unsuccessful.
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