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For the isolation and enumeration of bacteria, each water sample was serially diluted in sterile distilled water and plated on nutrient agar and different selective media.
The CellSearch system was used for the isolation and enumeration of CTCs from each 7.5 ml of blood separately.
The CellSearch system was used for the isolation and enumeration of CTCs, and 7.5 mL of the 10 mL in the tubes were assessed by this assay.
The CellSearch System (Veridex LLC, Raritan, NJ, USA) was used for the isolation and enumeration of circulating tumour cells (CTCs), which were defined as nucleated cells lacking cluster of differentiation 45 and expressing cytokeratin.
The CellSearch System (Veridex, LLC, Warren, NJ, USA) was used for the isolation and enumeration of CTCs from each 7.5 ml of blood separately using specific morphological criteria to identify CTCs (Allard et al, 2004).
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New technology allows the isolation and enumeration of circulating tumour cells (CTC) in patients with advanced prostate cancer.
The method employs galE− host bacteria to facilitate the isolation and enumeration of mutant copies of the lacZ transgene [ Gossen et al., 1992].
Defined numbers of cells from the 2 control lines (K562 and MDAMB231) were added to HNV blood in triplicate for analysis by the CellSearch system employing instrumental methods similar to those used routinely in the isolation and enumeration of CTCs from cancer patient blood samples.
The approach developed in this study was valuable for isolation and enumeration of chiA-positive bacteria in environmental samples.
MacConkey agar has been widely used as selective medium for isolation and enumeration of E. coli due to its low cost and high selectivity [ 2- 4].
Inoculant improvement has been associated to more effective methods to evaluate the quality of bacterial formulations (inoculants) (Tong and Sadowsky, 1994), as well as those related with the isolation, identification and enumeration of bacteria in carriers, seeds, soil and plants.
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